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The Gibco LV- MAX Transfection Kit is an essential component of the LV-MAX Lentiviral Production System.It is formulated for the transient transfection of Gibco Viral Production Cells (high-density suspension-adapted human embryonic kidney (HEK) 293-derived cells). The included high-efficiency, cationic, lipid-based transfection reagent, lentiviral .
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Here, we provide detailed protocols for the generation of CD4- and CD8-targeted LVs (which takes ~9 d in total). We also describe how to humanize immunodeficient mice with hematopoietic.
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